Plasmid

Part:BBa_K4357004:Design

Designed by: Di Yu   Group: iGEM22_UM_Macau   (2022-10-12)


Pasr-mCherry-pSB1C3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3110
    Illegal suffix found in sequence at 1062
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3110
    Illegal SpeI site found at 1063
    Illegal PstI site found at 1077
    Illegal NotI site found at 881
    Illegal NotI site found at 1070
    Illegal NotI site found at 3116
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3110
    Illegal XhoI site found at 890
    Illegal XhoI site found at 2094
    Illegal XhoI site found at 2986
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3110
    Illegal suffix found in sequence at 1063
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3110
    Illegal XbaI site found at 3125
    Illegal SpeI site found at 1063
    Illegal PstI site found at 1077
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to examine the Pasr promoter, a mcherry fluorecent sigal protein was add to express.


Source

https://parts.igem.org/Part:BBa_K1231000 https://parts.igem.org/Part:BBa_B0015 https://parts.igem.org/Part:BBa_K4357003 https://parts.igem.org/Part:BBa_B0034 https://parts.igem.org/Part:pSB1C3


References